Tuesday, December 31, 2019

Private Schools vs. Public Schools Sports Teams - 1606 Words

The imbalance between public and private school sports teams does not need to be adjusted. Private schools are offering an extra service that public schools cannot offer. That service is better teachers all the way to better coaches. There are no rules on how they can raise money for their athletic programs unlike public schools. Because of the athletic success more student athletes want to go to the private schools. Which in turn gives the student athletic a better opportunity to be recruited. No the private schools do not need to be adjusted public schools need to improve their outlook on athletics. There is one thing that a lot of public schools do very well is make them magnet schools for arts, drama and science but they never come†¦show more content†¦If a school has 1000 students with the multiplier the nonpublic school would be at 1500 forcing the school to compete against the bigger schools. (Popke, 2006) With this system in place they feel that this the only way to level the playing field. Another state Tennessee has used the multiplier policy and a separate division for all private and non-public schools that offer financial aid to the varsity athletes. For the nonpublic schools that do not render financial aid can still can compete in division I but to make the playing field even they must multiply the enrollment by 1.8 which moves them up a class. (Popke, 2012) Programs recruit themselves When you look at the success of private schools in athletics there is no need to recruit students. Just like a public magnet high school students come there because of the special services they offer. This is where the public schools need to adapt their way of thinking. The question is why students are leaving public schools to attend private schools. Lets start with coaches at a public high school. With the budget cuts more coaches are leaving public high schools this makes it hard for athletic directors to hire the right coach. (Milligen, 2013) This plays a factor for students when having the option to go to a private school. Teaches that are also coaches find it harder to do both jobs because of the cutbacks inShow MoreRelatedEssay about Home Schooling vs Public Schooling : Which One Is Better ?1102 Words   |  5 PagesHome Schooling vs. Public Schools: Which One is Better? Thesis Statement: Many parents question what is best for their child’s education, home schooling or public school. Home schooling can sometimes provide a more direct and focused education than public schools, Public School is better in many ways including the child’s social development. I. Home Schooling vs. Public Schooling II. The Pros and Cons of Home Schooling A. Home schooling can benefit both students and parents by providingRead More Private Education vs Public Education Essay817 Words   |  4 PagesPrivate Education vs Public Education Public Education v/s Private Education A person’s education is one of the most important factors in determining whether or not they will become a productive member of society. That is why when considering an education the quality of the education is almost as important as the education itself. So when private schools came into the picture the debate then began between public and private schools. Facing the technological revolution that we are goingRead MoreHomeschooling vs. Public School Essay1409 Words   |  6 PagesHomeschooling vs. Public School Homeschooling vs. public school has been a public debate for quite a while now. Some parents choose homeschooling because of the protection their child gains and some choose public education because of the atmosphere. 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A school and university have their own dress code which does not require a particular dress. Some researcher and scholar suggest misbehavior in the student are the result of banning uniform. But misbehavior is what they acquired in their life. Banning uniform, student can exercise their right to express themselves freely. Student will be able to focus on education and saveRead MoreFrom the Comfort of Your Own School1622 Words   |  7 PagesSomehow it seems that they are always the ones to win the spelling bees. They make other high school kids look E-L-E-M-E-N-T-A-R-Y. These students succeed because they were exposed to a better learning environment, were able to progress at their own pace, and had the freedom to choose their own studies. These kids are home schooled kids, and their style of education is more beneficial than public schooling. Home schooling is an educational system based at the home. Here, a child learns at theirRead MoreHome School Vs. Public School1638 Words   |  7 PagesHome School vs. Public School There has been an ongoing discussion between parents and scientists about public school and home school. While homeschooling continues to grow in popularity, many people still have a negative view of it and it is not uncommon, even for friends and family, to criticize those who choose to home school their kids. Some say homeschooling your child will further them in their academic pursuits but hinder them in their social life. Each situation is different for each person

Monday, December 23, 2019

Legalism And Confucianism The Fundamental Basis Of...

Legalism and Confucianism form the fundamental basis of traditional Chinese culture and has exercised extensive influence over Chinese domestic laws for a period of almost 1,400 years. The Tang dynasty of 619 to 907 especially marked a period of efficient government, strong economic growth and effective administration in all forms of social and legal affairs. The amalgamation of two prominent Chinese philosophies amounted to immense success by creating a framework of domestic laws that was a partnership between the Confucian orthodox ideology and a structurally Legalist state. Such a system of legal code which eventually shaped contemporary social, ethical and political aspects of Chinese culture, allowed for Confucianism’s emphasis on†¦show more content†¦Supplementarily, the influence of Legalism’s FÇŽ (æ ³â€¢, law or principle), Shà ¹ (è ¡â€œ, method, tactic, or art) and Shà ¬ (å‹ ¢, legitimacy, power, or charisma) on the Code is reflected in its ad ministrative focus and the linkage of behaviour with a gradation of punishments. The collective state doctrine of Confucianism’s moral objective and Legalism’s administrative focus overall became conducive to the consolidation and preservation of an ethical society. The various commentary and assertions made by historians confirms not only the remarkable impact Confucianism and Legalism had on the Tang’s domestic laws but also affirms the distinction of perspectives on which philosophy primarily dominated these laws. Professor Norman P. Ho with a certificate in Chinese History at Peking University and a Juris Doctor Degree from New York University School of Law reviews the practice of Confucian jurisprudence in an expression of the excellence of the Tang’s legal code. Ying Lin with a Bachelor of Laws from ShenZhen and a Master of Laws from the University of Wollongong in contrast examines the duality of Confucian morality and Legalist rule of law despite their adversarial nature. ‘Law and Morality: Some Reflections on the Chinese Experience Past and Present’ by Herbert H. P. Ma addresses the domination of Confucianism in Tang’s laws and Legalism’s existence as only a foundation inShow MoreRelatedNegative Effects Of The Han Dynasty951 Words   |  4 PagesThroughout the development of Chinese history, dynasties or ruling families, have had major effects on China’s social, political and economic systems. For instance, several notable dynasties include the Zhou, Qin, and Han dynasty. Nevertheless, the sentiment favoring a certain dynasty led to debate between individuals. Thus, several individuals avouch that the Han dynasty had the most profound impact on the development of China, while others argue that it was the Qin dynasty who had a greater effectRead MoreHow Law is Made and Interpreted in France, China and Indonesia2652 Words   |  11 PagesOutline How Law is Made and Interpreted in French Civil Law System French Civil Code of 1804 Sources of French Civil Law Doctrine How Law is Made and Interpreted in China Confucianism Legalism How Sharia Law is Made and Interpreted in Pluralist Indonesian System How Law is Made and Interpreted in French Civil Law System The civil law is a branch of private law governing relations between people, whether individuals or legal entities (Sacco, 1991, p.25). It comprises of: 1. TheRead MoreComparing Empires Rome and China3033 Words   |  13 PagesComparing Empires: Roman and Chinese Consolidating the Roman and Chinese Empires 1. both empires defined themselves in universal terms 2. both invested heavily in public works 3. both claimed supernatural sanctions a. deceased Roman emperors as gods (imperial cult) i. persecution of Christians for nonparticipation in cult b. Chinese emperor as Son of Heaven i. rule by Mandate of Heaven ii. dependent on just rule iii. heavy ritual duties to maintain relationship between earth and heaven iv. moralRead MoreBranches of Philosophy8343 Words   |  34 PagesBranches of philosophy The following branches are the main areas of study: †¢ Metaphysics investigates the nature of being and the world. Traditional branches are cosmology and ontology. †¢ Epistemology is concerned with the nature and scope of knowledge, and whether knowledge is possible. Among its central concerns has been the challenge posed by skepticism and the relationships between truth, belief, and justification. †¢ Ethics, or moral philosophy, is concerned with questions of how

Sunday, December 15, 2019

Second Foundation 16. Beginning of War Free Essays

string(39) " to stare with force at the young man\." For reason or reasons unknown to members of the Galaxy at the time of the era under discussion, Intergalactic Standard Time defines its fundamental unit, the second, as the time in which light travels 299,776 kilometers. 86,400 seconds are arbitrarily set equal to one Intergalactic Standard Day; and 365 of these days to one Intergalactic Standard Year. Why 299,776?- Or 86,400?- Or 365? Tradition, says the historian, begging the question. We will write a custom essay sample on Second Foundation 16. Beginning of War or any similar topic only for you Order Now Because of certain and various mysterious numerical relationships, say the mystics, cultists, numerologists, metaphysicists. Because the original home-planet of humanity had certain natural periods of rotation and revolution from which those relationships could be derived, say a very few. No one really knew. Nevertheless, the date on which the Foundation cruiser, the Hober Mallow met the Kalganian squadron, headed by the Fearless, and, upon refusing to allow a search party to board, was blasted into smoldering wreckage was 185; 11692 G.E. That is, it was the 185th day of the 11,692nd year of the Galactic Era which dated from the accession of the first Emperor of the traditional Kamble dynasty. It was also 185; 419 A.S. – dating from the birth of Seldon – or 185; 348 Y.F. – dating from the establishment of the Foundation. On Kalgan it was 185; 56 F.C. – dating from the establishment of the First Citizenship by the Mule. In each case, of course, for convenience, the year was so arranged as to yield the same day number regardless of the actual day upon which the era began. And, in addition, to all the millions of worlds of the Galaxy, there were millions of local times, based on the motions of their own particular heavenly neighbors. But whichever you choose: 185; 11692-419-348-56 – or anything – it was this day which historians later pointed to when they spoke of the start of the Stettinian war. Yet to Dr. Darell, it was none of these at all. It was simply and quite precisely the thirty-second day since Arcadia had left Terminus. What it cost Darell to maintain stolidity through these days was not obvious to everyone. But Elvett Semic thought he could guess. He was an old man and fond of saying that his neuronic sheaths had calcified to the point where his thinking processes were stiff and unwieldy. He invited and almost welcomed the universal underestimation of his decaying powers by being the first to laugh at them. But his eyes were none the less seeing for being faded; his mind none the less experienced and wise, for being no longer agile. He merely twisted his pinched lips and said, â€Å"Why don’t you do something about it?† The sound was a physical jar to Darell, under which he winced. He said, gruffly, â€Å"Where were we?† Semic regarded him with grave eyes. â€Å"You’d better do something about the girl.† His sparse, yellow teeth showed in a mouth that was open in inquiry. But Darell replied coldly, â€Å"The question is: Can you get a Symes-Molff Resonator in the range required?† Well, I said I could and you weren’t listening-â€Å" â€Å"I’m sorry, Elvett. It’s like this. What we’re doing now can be more important to everyone in the Galaxy than the question of whether Arcadia is safe. At least, to everyone but Arcadia and myself, and I’m willing to go along with the majority. How big would the Resonator be?† Semic looked doubtful, â€Å"I don’t know. You can find it somewheres in the catalogues.† â€Å"About how big. A ton? A pound? A block long?† â€Å"Oh, I thought you meant exactly. It’s a little jigger.† He indicated the first joint of his thumb. â€Å"About that.† â€Å"All right, can you do something like this?† He sketched rapidly on the pad he held in his lap, then passed it over to the old physicist, who peered at it doubtfully, then chuckled. â€Å"Y’know, the brain gets calcified when you get as old as I am. What are you trying to do?† Darell hesitated. He longed desperately, at the moment, for the physical knowledge locked in the other’s brain, so that he need not put his thought into words. But the longing was useless, and he explained. Semic was shaking his head. â€Å"You’d need hyper-relays. The only things that would work fast enough. A thundering lot of them.† â€Å"But it can be built?† â€Å"Well, sure.† â€Å"Can you get all the parts? I mean, without causing comment? In line with your general work.† Semic lifted his upper lip. â€Å"Can’t get fifty hyper-relays? I wouldn’t use that many in my whole life.† â€Å"We’re on a defense project, now. Can’t you think of something harmless that would use them? We’ve got the money.† â€Å"Hm-m-m. Maybe I can think of something.† â€Å"How small can you make the whole gadget?† â€Å"Hyper-relays can be had micro-size†¦ wiring†¦ tubes – Space, you’ve got a few hundred circuits there.† â€Å"I know. How big?† Semic indicated with his hands. â€Å"Too big,† said Darell. â€Å"I’ve got to swing it from my belt† Slowly, he was crumpling his sketch into a tight ball. When it was a hard, yellow grape, he dropped it into the ash tray and it was gone with the tiny white flare of molecular decomposition. He said, â€Å"Who’s at your door?† Semic leaned over his desk to the little milky screen above the door signal. He said, â€Å"The young fellow, Anthor. Someone with him, too.† Darell scraped his chair back. â€Å"Nothing about this, Semic, to the others yet. It’s deadly knowledge, if they find out, and two lives are enough to risk.† Pelleas Anthor was a pulsing vortex of activity in Semic’s office, which, somehow, managed to partake of the age of its occupant. In the slow turgor of the quiet room, the loose, summery sleeves of Anthor’s tunic seemed still a-quiver with the outer breezes. He said, â€Å"Dr. Darell, Dr. Semic – Orum Dirige.† The other man was tall. A long straight nose that lent his thin face a saturnine appearance. Dr. Darell held out a hand. Anthor smiled slightly. â€Å"Police Lieutenant Dirige,† he amplified. Then, significantly, â€Å"Of Kalgan.† And Darell turned to stare with force at the young man. You read "Second Foundation 16. Beginning of War" in category "Essay examples" â€Å"Police Lieutenant Dirige of Kalgan,† he repeated, distinctly. â€Å"And you bring him here. Why?† â€Å"Because he was the last man on Kalgan to see your daughter. Hold, man.† Anthor’s look of triumph was suddenly one of concern, and he was between the two, struggling violently with Darell. Slowly, and not gently, he forced the older man back into the chair. â€Å"What are you trying to do?† Anthor brushed a lock of brown hair from his forehead, tossed a hip lightly upon the desk, and swung a leg, thoughtfully. â€Å"I thought I was bringing you good news.† Darell addressed the policeman directly, â€Å"What does he mean by calling you the last man to see my daughter? Is my daughter dead? Please tell me without preliminary.† His face was white with apprehension. Lieutenant Dirige said expressionlessly, † ‘Last man on Kalgan’ was the phrase. She’s not on Kalgan now. I have no knowledge past that.† â€Å"Here,† broke in Anthor, â€Å"let me put it straight. Sorry if I overplayed the drama a bit, Doc. You’re so inhuman about this, I forget you have feelings. In the first place, Lieutenant Dirige is one of us. He was born on Kalgan, but his father was a Foundation man brought to that planet in the service of the Mule. I answer for the lieutenant’s loyalty to the Foundation. â€Å"Now I was in touch with him the day after we stopped getting the daily report from Munn-â€Å" â€Å"Why?† broke in Darell, fiercely. â€Å"I thought it was quite decided that we were not to make a move in the matter. You were risking their lives and ours.† â€Å"Because,† was the equally fierce retort, â€Å"I’ve been involved in this game for longer than you. Because I know of certain contacts on Kalgan of which you know nothing. Because I act from deeper knowledge, do you understand?† â€Å"I think you’re completely mad.† â€Å"Will you listen?† A pause, and Darell’s eyes dropped. Anthor’s lips quirked into a half smile, â€Å"All right, Doc. Give me a few minutes. Tell him, Dirige.† Dirige spoke easily: â€Å"As far as I know, Dr. Darell, your daughter is at Trantor. At least, she had a ticket to Trantor at the Eastern Spaceport. She was with a Trading Representative from that planet who claimed she was his niece. Your daughter seems to have a queer collection of relatives, doctor. That was the second uncle she had in a period of two weeks, eh? The Trantorian even tried to bribe me – probably thinks that’s why they got away.† He smiled grimly at the thought. â€Å"How was she?† â€Å"Unharmed, as far as I could see. Frightened. I don’t blame her for that. The whole department was after her. I still don’t know why.† Darell drew a breath for what seemed the first time in several minutes. He was conscious of the trembling of his hands and controlled them with an effort. â€Å"Then she’s all right. This Trading Representative, who was he? Go back to him. What part does he play in it?† â€Å"I don’t know. Do you know anything about Trantor?† â€Å"I lived there once.† â€Å"It’s an agricultural world, now. Exports animal fodder and grains, mostly. High quality! They sell them all over the Galaxy. There are a dozen or two farm co-operatives on the planet and each has its representatives overseas. Shrewd sons of guns, too- I knew this one’s record. He’d been on Kalgan before, usually with his wife. Perfectly honest. Perfectly harmless.† â€Å"Um-m-m,† said Anthor. â€Å"Arcadia was born in Trantor, wasn’t she, Doc?† Darell nodded. â€Å"It hangs together, you see. She wanted to go away – quickly and far – and Trantor would suggest itself. Don’t you think so?† Darell said: â€Å"Why not back here?† â€Å"Perhaps she was being pursued and felt that she had to double off in a new angle, eh?’ Dr. Darell lacked the heart to question further. Well, then, let her be safe on Trantor, or as safe as one could be anywhere in this dark and horrible Galaxy. He groped toward the door, felt Anthor’s light touch on his sleeve, and stopped, but did not turn. â€Å"Mind if I go home with you, Doc?† â€Å"You’re welcome,† was the automatic response. By evening, the exteriormost reaches of Dr. Darell’s personality, the ones that made immediate contact with other people had solidified once more. He had refused to eat his evening meal and had, instead, with feverish insistence, returned to the inchwise advance into the intricate mathematics of encephalographic analysis. It was not till nearly midnight, that he entered the living room again. Pelleas Anthor was still there, twiddling at the controls of the video. The footsteps behind him caused him to glance over his shoulder. â€Å"Hi. Aren’t you in bed yet? I’ve been spending hours on the video, trying to get something other than bulletins. It seems the F.S. Hober Mallow is delayed in course and hasn’t been heard from† â€Å"Really? What do they suspect?† â€Å"What do you think? Kalganian skulduggery. There are reports that Kalganian vessels were sighted in the general space sector in which the Hober Mallow was last heard from?† Darell shrugged, and Anthor rubbed his forehead doubtfully. â€Å"Look doc,† he said, â€Å"why don’t you go to Trantor?† â€Å"Why should I?† â€Å"Because â€Å"You’re no good to us here. You’re not yourself. You can’t be. And you could accomplish a purpose by going to Trantor, too. The old Imperial Library with the complete records of the Proceedings of the Seldon Commission are there-â€Å" â€Å"No! The Library has been picked clean and it hasn’t helped anyone.† â€Å"It helped Ebling Mis once.† â€Å"How do you know? Yes, he said he found the Second Foundation, and my mother killed him five seconds later as the only way to keep him from unwittingly revealing its location to the Mule. But in doing so, she also, you realize, made it impossible ever to tell whether Mis really did know the location. After all, no one else has ever been able to deduce the truth from those records.† â€Å"Ebling Mis, if you’ll remember, was working under the driving impetus of the Mule’s mind.† â€Å"I know that, too, but Mis’ mind was, by that very token, in an abnormal state. Do you and I know anything about the properties of a mind under the emotional control of another; about its abilities and shortcomings? In any case, I will not go to Trantor.† Anthor frowned, â€Å"Well, why the vehemence? I merely suggested it as – well, by Space, I don’t understand you. You look ten years older. You’re obviously having a hellish time of it. You’re not doing anything of value here. If I were you, I’d go and get the girl.† â€Å"Exactly! It’s what I want to do, too. That’s why I won’t do it. Look, Anthor, and try to understand. You’re playing – we’re both playing – with something completely beyond our powers to fight. In cold blood, if you have any, you know that, whatever you may think in your moments of quixoticism. â€Å"For fifty years, we’ve known that the Second Foundation is the real descendent and pupil of Seldonian mathematics. What that means, and you know that, too, is that nothing in the Galaxy happens which does not play a part in their reckoning. To us, all life is a series of accidents, to be met with by improvisations To them, all life is purposive and should be met by precalculation. â€Å"But they have their weakness. Their work is statistical and only the mass action of humanity is truly inevitable. Now how I play a part, as an individual, in the foreseen course of history, I don’t know. Perhaps I have no definite part, since the Plan leaves individuals to indeterminacy and free will. But I am important and they – they, you understand – may at least have calculated my probable reaction. So I distrust, my impulses, my desires, my probable reactions. â€Å"I would rather present them with an improbable reaction. I will stay here, despite the fact that I yearn very desperately to leave.*** â€Å"No! Because I yearn very desperately to leave.† The younger man smiled sourly. â€Å"You don’t know your own mind as well as they might. Suppose that – knowing you – they might count on what you think, merely think, is the improbable reaction, simply by knowing in advance what your line of reasoning would be.† â€Å"In that case, there is no escape. For if I follow the reasoning you have just outlined and go to Trantor, they may have foreseen that, too. There is an endless cycle of double-double-double-double-crosses. No matter how far I follow that cycle, I can only either go or stay. The intricate act of luring my daughter halfway across the Galaxy cannot be meant to make me stay where I am, since I would most certainly have stayed if they had done nothing. It can only be to make me move, and so I will stay. â€Å"And besides, Anthor, not everything bears the breath of the Second Foundation; not all events are the results of their puppeting. They may have had nothing to do with Arcadia’s leave-taking, and she may be safe on Trantor when all the rest of us are dead.† â€Å"No,† said Anthor, sharply, â€Å"now you are off the track.† â€Å"You have an alternative interpretation?† â€Å"I have – if you’ll listen.† â€Å"Oh, go ahead. I don’t lack patience.† â€Å"Well, then – how well do you know your own daughter?† â€Å"How well can any individual know any other? Obviously, my knowledge is inadequate.† â€Å"So is mine on that basis, perhaps even more so – but at least, I viewed her with fresh eyes. Item one: She is a ferocious little romantic, the only child of an ivory-tower academician, growing up in an unreal world of video and book-film adventure. She lives in a weird self-constructed fantasy of espionage and intrigue. Item two: She’s intelligent about it; intelligent enough to outwit us, at any rate. She planned carefully to overhear our first conference and succeeded. She planned carefully to go to Kalgan with Munn and succeeded. Item three: She has an unholy hero-worship of her grandmother – your mother – who defeated the Mule. â€Å"I’m right so far, I think? All right, then. Now, unlike you, I’ve received a complete report from Lieutenant Dirige and, in addition, my sources of information on Kalgan are rather complete, and all sources check. We know, for instance, that Homir Munn, in conference with the Lord of Kalgan was refused admission to the Mule’s Palace, and that this refusal was suddenly abrogated after Arcadia had spoken to Lady Callia, the First Citizen’s very good friend.† Darell interrupted. â€Å"And how do you know all this?† â€Å"For one thing, Munn was interviewed by Dirige as part of the police campaign to locate Arcadia. Naturally, we have a complete transcript of the questions and answers. â€Å"And take Lady Callia herself. It is rumored that she has lost Stettin’s interest, but the rumor isn’t borne out by facts. She not only remains unreplaced; is not only able to mediate the lord’s refusal to Munn into an acceptance; but can even engineer Arcadia’s escape openly. Why, a dozen of the soldiers about Stettin’s executive mansion testified that they were seen together on the last evening. Yet she remains unpunished. This despite the fact that Arcadia was searched for with every appearance of diligence.† â€Å"But what is your conclusion from all this torrent of ill-connection?† â€Å"That Arcadia’s escape was arranged.† â€Å"As I said.† â€Å"With this addition. That Arcadia must have known it was arranged; that Arcadia, the bright little girl who saw cabals everywhere, saw this one and followed your own type of reasoning. They wanted her to return to the Foundation, and so she went to Trantor, instead. But why Trantor?† â€Å"Well, why?† â€Å"Because that is where Bayta, her idolized grandmother, escaped when she was in flight. Consciously or unconsciously, Arcadia imitated that. I wonder, then, if Arcadia was fleeing the same enemy.† â€Å"The Mule?† asked Darell with polite sarcasm. â€Å"Of course not. I mean, by the enemy, a mentality that she could not fight. She was running from the Second Foundation, or such influence thereof as could be found on Kalgan.† â€Å"What influence is this you speak of?† â€Å"Do you expect Kalgan to be immune from that ubiquitous menace? We both have come to the conclusion, somehow, that Arcadia’s escape was arranged. Right? She was searched for and found, but deliberately allowed to slip away by Dirige. By Dirige, do you understand? But how was that? Because he was our man. But how did they know that? Were they counting on him to be a traitor? Eh, doc?† â€Å"Now you’re saying that they honestly meant to recapture her. Frankly, you’re tiring me a bit, Anthor. Finish your say; I want to go to bed.† â€Å"My say is quickly finished.† Anthor reached for a small group of photo-records in his inner pocket. It was the familiar wigglings of the encephalograph. â€Å"Dirige’s brainwaves,† Anthor said, casually, â€Å"taken since he returned.† It was quite visible to Darell’s naked eye, and his face was gray when he looked up. â€Å"He is Controlled.† â€Å"Exactly. He allowed Arcadia to escape not because he was our man but because he was the Second Foundation’s.† â€Å"Even after he knew she was going to Trantor, and not to Terminus.† Anthor shrugged. â€Å"He had been geared to let her go. There was no way he could modify that. He was only a tool, you see. It was just that Arcadia followed the least probable course, and is probably safe. Or at least safe until such time as the Second Foundation can modify the plans to take into account this changed state of affairs-â€Å" He paused. The little signal light on the video set was flashing. On an independent circuit, it signified the presence of emergency news. Darell saw it, too, and with the mechanical movement of long habit turned on the video. They broke in upon the middle of a sentence but before its completion, they knew that the Hober Mallow, or the wreck thereof, had been found and that, for the first time in nearly half a century, the Foundation was again at war. Anthor’s jaw was set in a hard line. â€Å"All right, doc, you heard that. Kalgan has attacked; and Kalgan is under the control of the Second Foundation. Will you follow your daughter’s lead and move to Trantor?† â€Å"No. I will risk it. Here.† â€Å"Dr. Darell. You are not as intelligent as your daughter. I wonder how far you can be trusted.† His long level stare held Darell for a moment, and then without a word, he left. And Darell was left in uncertainty and – almost – despair. Unheeded, the video was a medley of excited sight-sound, as it described in nervous detail the first hour of the war between Kalgan and the Foundation. How to cite Second Foundation 16. Beginning of War, Essay examples

Saturday, December 7, 2019

Linear Statistical Models Regression Analysis MyAssignmenthelp.com

Question: Discuss about the Linear Statistical Models Regression Analysis. Answer: Introduction The purpose of this study is determine the impact of particular population characteristics on health development. It seeks to determine some major aspects of the population based on the amount of expenditure set aside for health. Health development is among the most significant worldwide concerns as it involves and affects everyone. The matter also significantly affects other aspects such as population, economy, etc. It is for this reason that researchers, governments and business people have made an effort of trying to know the performance of the health sector in their countries, and other countries. Studies to determine the health development have been rapidly done between the years 2001 and 2015, across several aspects of the health sector such as birth rate, fertility rate, health expenditure, death rate, etc. Recently World Bank released data on health development in the past 15 years containing the health and population statistics. This data provides a rough estimate of health development across various countries, through fifteen years. Governments need to know how much they have been investing in health and try to predict what they will have to invest in the coming future from the data, in order to keep a good balance of the population. Businesses should also be able to know the percentage that people are willing to invest in their health and how they can create healthcare solutions. Clustering is a practice used in data analysis which involves gathering bunch of objects and separating them into sensible groupings of similar objects. This is done by determining how they are similar and how they are different.Jain (2010) explains clustering analysis is a study that deals with the methods and algorithms for clustering objects on the basis of some perceived or measured similarity. It does not use category labels that group objects with previous known identifiers. K-means clustering K-means clustering is a type of data clustering commonly used for performing unsupervised learning tasks (Ding He 2004, pg. 29) This kind of clustering takes some point in space and put them into k groups (k is the number of groups you want). Since we know that there are 7 countries included in the sample, we created an algorithm code that would group the data into 7 cluster. table(mydataCluster$cluster, mydata$Country) As we can see, the data belonging to theASM got grouped into cluster 5,AUSinto cluster 1, FJI into cluster 4, MHL into cluster 3. The algorithm wrongly classified three data points belonging toMNGand four data points belonging toWSM. Regression analysis describes the relationships between two variables in a simplified mathematical model (Schneider, Hommel Blettner 2010, p. 776). It predicts one variable based on other variable(s). In this case, linear regression, death rate is used as the dependent variable (Y), and health expenditure as the independent variable (X). The prediction of death rate based on health expenditure is done using the linear model of the form: Simple linear regression analysis is performed on the two variables at 95% confidence level. Performing linear regression of year as an independent variable and birth rate as a dependent variable. We will therefore use the variable year, to predict birth rate using the equation: #linear regression of death rate verses health expenditure Conclusion The first regression model has a p-value less than 0.05, this means that the model is significant and can therefore predict death rate based on health expenditure. The model however bears a low coefficient of determinant, of 0.03429, this means that the predictor variable only explains 3.4% of the variation in death rate. For the second regression model, the p-value also results in a conclusion that the model is significant and can predict birth rate based on health expenditure. The model has a slightly lower coefficient of determinant than the previous model. The R-squared value of 0.0209 implies that only 2.09% of the variation in birth rate is explained by health expenditure. Further studies should therefore be made to determine the other variables affecting these health development factors. A major difficulty when performing the analysis was the fact that the data set was in a wide format and had to convert it to be in a long format to ease analysis. Another challenge was the fact that attributes were in rows instead of columns so in that case I had to convert the data to wide format. Finally this dataset had a lot of missing values which made it difficult to do the analysis, this prompt the use of na.rm method in R when performing most analysis. From this challenges I learnt how to convert dataset from one format to another lets say wide to long or vice versa also how to use functions to omit missing values (N/A) in analysis. In future analysis I will ensure that data is well structured and ready for analysis before starting the analysis. Reference Schneider, A., Hommel, G. and Blettner, M., 2010. Linear Regression Analysis.Dtsch Rztebl Int,107(44), pp.776-82. Neter, J., Kutner, M.H., Nachtsheim, C.J. and Wasserman, W., 1996.Applied linear statistical models(Vol. 4, p. 318). Chicago: Irwin. Jain, A.K., 2010. Data clustering: 50 years beyond K-means.Pattern recognition letters,31(8), pp.651-666. Ding, C. and He, X., 2004, July. K-means clustering via principal component analysis. InProceedings of the twenty-first international conference on Machine learning(p. 29). ACM.

Friday, November 29, 2019

Basic Input/Output System Essay Example

Basic Input/Output System Paper If Microsoft Windows (MS Windows), a well-known operating system, become attractive interface between human/user with computer, then BIOS is a heart that work unconsciously to collect several information that a user input. BIOS (Basic Input/Output System) are codes that have critical functions in computer system such as collecting button taps from keyboard or laying the pixel to the screen. This little program work behind the screen, translate MS Windows commands that people know them as representation of zero and one numeric, which only known by computer hardware. Like a heart role in human body, BIOS also has a strong influence to computer works. We will write a custom essay sample on Basic Input/Output System specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Basic Input/Output System specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Basic Input/Output System specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Since computer system needs refreshment, the BIOS also need it. However, to update BIOS is easy although user must perform it carefully since any fault will lead computer to misconduct. The affecting are: the real correct BIOS updating will make a computer progressively stabilize and in line with the computer hardware’s, but if there is a little mistake, users just can bewail the computer’s motherboard since it cannot be used anymore. Therefore, when updating BIOS, make sure to read all the instructions in the readme BIOS update file and always do as the instructions, which are searchable in BIOS website. I. Basic Input/Output System (BIOS) I.1. Understanding BIOS BIOS stand for Basic Input/Output System. This is a program, which also known as firmware that is stored in a ROM (Read Only Memory) chip on the motherboard, commonly referred to as the BIOS chip. The BIOS has critical responsibility since it detects, tests, initializes and monitors the system hardware from the moment the system is turn on until Windows takes over. When a user turns on the computer on, the BIOS instantly identifies all of the onboard devices such as processor, memory, video card, etc and then runs the Power on Self Test (P.O.S.T) to determine whether each detected device is working properly. If all of the devices pass the POST tests then BIOS initializes each device, detects the hard drives, CD-ROM and floppy drives and then searches the first boot device (normally the hard drive or floppy drive) for files necessary to load the operating system software and then passes control of the system to the operating system software. If any devices fail the tests, the POST provides an error message, which is displayed on the computer screen or it provides a series of beep sounds to computer’s speakers to indicate there is a problem. The sequence of beeps can be used to identify exactly what the problem is. I.2 Importance of BIOS A flash BIOS chip contains BIOS information that can be erasing and updating. This allows it to recognize new features on an upgraded processor chip or new component that has come out since bought r computer. Occasionally, a computer will need to have its BIOS updated. This is especially true of older machines. As new devices and standards arise, the BIOS needs to change in order to understand the new hardware identification. Since the BIOS are stored in some form of ROM, changing the BIOS is a bit harder than upgrading most other types of software (Biosman, 2006). Most commonly, a user would want to upgrade BIOS on an older machine so that it can use Plug and Play features built into Windows.

Monday, November 25, 2019

The Effects of TiVo On Advertising †Marketing Essay

The Effects of TiVo On Advertising – Marketing Essay Free Online Research Papers The Effects of TiVo On Advertising Marketing Essay Putting â€Å"TiVo† and â€Å"commercial† next to each other may seem paradoxical. TiVo is initially selling its feature of skipping commercials. However, with the launch of TiVo, many advertisers feel threatened for their commercials not being broadcasted to what they had paid for. In order not anger major supporters of TV programs, TiVo has been trying to be commercial-friendly and has eliminated the feature of skipping commercials. In the August issue of Television Week, TiVo recently signs contract with five brands to advertise. The proposed advertising method is a branded tag on TiVo consumers’ screens, and they can find out more information by pressing a button on their remote. This â€Å"branded tag† operates similar to Internet advertising. Consumers are exposed to promotional messages but not forced to watch them. Given its similarity to Internet advertising, advertisers can offer incentives for people to click the branded tags. For example, TiVo could count the number of clicks in a certain designated period. For each click of the tag, advertisers would offer certain amounts of monetary reimbursement to the consumer. Or the reimbursement could be used as credit in paying the monthly TiVo subscription fee. Advertisers could also give exclusive offers to only those who clicked the tag. Money is usually the best incentive to get people to do an act. TiVo now has 3.3 million users. Assume they all clicked the tag for Tylenol once in a month, and each click is worth $0.45. Each month Tylenol would have to send out $1,685,000 of reimbursement, and $20,220,000 for a year. That is only about 2.6% of its total sale of $786.5 million sales in 2004. Assume all TiVo viewers on average $6 on Tylenol products in the month within clicking the tag, Tylenol makes a profit of $5.55 per viewer after issuing the reimbursement. Therefore, Tylenol still makes $18,315,000 for the month. Even though it has to pay consumers to watch its commercial, Tylenol is not losing money. Consumers would be happy to watch commercials knowing that they are being paid to watch. Although consumers are willing to spend over $100 to avoid commercials, they are also willing to spend several seconds to make some money. Offering reimbursement should compensates consumers’ frustration for unable to avoid ads, yet advertisers still get their messages across. After all, Tom Rogers the CEO of TiVo sees TiVo â€Å"as a platform for dealing with, and allowing for the growth of, the advertising business.† Research Papers on The Effects of TiVo On Advertising - Marketing EssayMarketing of Lifeboy Soap A Unilever ProductAnalysis of Ebay Expanding into AsiaDefinition of Export QuotasThe Project Managment Office SystemTwilight of the UAWAnalysis Of A Cosmetics AdvertisementEffects of Television Violence on ChildrenIncorporating Risk and Uncertainty Factor in CapitalPETSTEL analysis of IndiaGenetic Engineering

Friday, November 22, 2019

Interview and National Political Research Paper

Interview and National Political - Research Paper Example National policy of a country is in line with its manifesto and its aspirations towards the global relationship with outside world. National policy serves in multiple ways; it allows protecting the sovereignty of the state, presenting the state’s ideology and most importantly enables engaging into dialogue and mediation with outside world for the betterment of its economic, social and political condition. Syria is one similar country that has a clearly defined national policy and foreign policy that allows it engaging into global relations. Based on its geo strategic importance Syria holds significance for number of reasons. These reasons stretch beyond geographic, social, political factors. It has gained importance in recent times due to the ongoing events in the region. The region in itself is of high importance considering the neighboring countries and the natural location. Keeping the global impact in view, the Arab Spring that took off from Tunisia, was one similar event w hich engulfed fire in the neighboring countries. The aim of Arab spring was to eliminate the status quo. Since Syria also fell in the line of fire courtesy Bashar Al Asad’s status quo existence, the fire of spring penetrated deep into Syrian towns and cities; triggering a civil war. The objective of this paper is to get a familiarity with the international politics and the world scenario. This is done through an interview conducting. The interview will allow understanding different facets of a research study along with global politics and geography comprehension. The paper is divided into three major sections, the first part deals with the geographic details of Syria, the second gives an insight into the form of government in place, while the third entails Syria’s relationship with United States of America. Brief overview of the country Syria is located in the Western Part of Asia. It is a Muslim populace country with different percentages of Shiite and Sunni followers amongst them. Other nations within Syria include Christians, Kurds and some traces of Turks are also visible in the country. Geographic location is of high importance and thereby makes it center of attention in the entire world. It is in the vicinity that includes Lebanon, Israel, and Turkey as its neighboring countries and their borders are mutually shared. Mediterranean Sea lies to its West and adds another point of importance on strategic front to its location. It also shares border with Jordan and Iraq making it one of the most entangled and attached country with other regional states. Modern day existence: The modern day existence stretches back to the end of World War 1 when the mandate system was introduced for the first time under the auspices of League of Nations. Syria was given under direct protection and guidance of France(Commins, 111). This was as a result of abolishing of Ottoman Empire. The second resurrection of Syria took place in 1946 (Darke , 12) right after Fir st World War when a sovereign republic came into existence. The size of Syria is 186,745 (square kilo meter). The total population of Syria is around 22 million. The characteristic of Syrian population is a multi cultural outlook consisting of Muslims and Christians in small amount. Syrian people are largely hospitable; they are fond of good food, traveling, outings in the local towns and cities. Their ways are depictive of Arabian culture, music is part of their culture, and so is art. Amongst the Muslims that constitute around 95

Wednesday, November 20, 2019

Paper Essay Example | Topics and Well Written Essays - 250 words - 13

Paper - Essay Example She worked at the Red Cross until 1966 when she joined state department as a nursing recruitment. Under this role, she set up the Community Nursing Services to help poor patients with home nursing. Mrs. Randolph served as the director of the Community Nursing Services from 1966 to 1970 and later became a community health programs supervisor for the state until she retired in 1980. Due to her exemplary service and passion for assisting poor people, she got several awards including American Association of Nurses’ leadership award. Additionally, she was an approved member of National Association for the Advancement of Colored People, American Association of Nurses, and American Civil Liberties Union. She was married to the late Lionel in 1944 with whom they had two sons, Lionel Randolph Jr. of Philadelphia, Pa and Jack Randolph of Bennington, Vt. She is leaves behind no grandchildren. On Friday at 10:00 a.m., there will be a service at All Saints Episcopal Church on 12th St. Thereafter; she will be buried at the All Saints Cemetery on the same street. Her family requests mourners to take flowers to the National Health Council instead of her funeral and grave. Her spirit of compassion and whole heartedness lives with us

Monday, November 18, 2019

Sociology and the culture Essay Example | Topics and Well Written Essays - 500 words

Sociology and the culture - Essay Example Multiculturalism can be defined as the notion that American society should be understood as a collection of diverse cultural groups rather than as a single, unified national body on one hand or as simply an aggregate of atomized individuals on the other. A multiculturalism sensibility implies that the government must recognize and respect if not nurture the diversity and integrity of racial and ethnic communities. The reason why different student groups define success in different ways is that different race and ethnical groups have sometimes their own understanding of their roles in American society which based on historical background and in some cases on public opinion. American society still operates in a paradigm in which an individual is a member of the "majority" or the "minority," either White or Non-White. This Black-Non White binary has influenced courts' and legislatures' race-conscious remedies such as school desegregation orders and employment discrimination claims. The White-Non-White paradigm is injurious, because instead of promoting equality, it promotes the dominance of whiteness. "White" becomes the singular point of reference for all other races; if one is not White, the "other" race to which one belongs is immaterial.

Saturday, November 16, 2019

Central african republic causes of poverty

Central african republic causes of poverty Introduction Poverty as defined by the United Nations is a denial of choices and opportunities, a violation of human dignity. It means lack of basic capacity to participate effectively in society. It means not having enough to feed and cloth a family, not having a school or clinic to go to, not having the land on which to grow ones food or a job to earn ones living, not having access to credit. It means insecurity, powerlessness and exclusion of individuals, households and communities. It means susceptibility to violence, and it often implies living on marginal or fragile environments, without access to clean water or sanitation (UN Statement, 1998). The major areas of the world that are prone to the evil of poverty are the countries of Africa and Asia. There are many countries in Africa where the people are not able to meet their one ends meal. We can also see from the figure below that especially in the central part of Africa or Sub-Saharan countries most of the people struggle very hard to earn even $3-$9 per day and to make their living. Central African Republic (CAR) which is a part of Sub-Saharan African countries is one those countries where people face this problem. Central African Republic CAR, a former French colony of Ubangi-Shari became the Central African Republic upon independence in 1960. The Central African Republic ranks 171 as a poor country. The Central African Republic is classified as one of the worlds least developed countries, with an estimated annual per capita income of $310 (2000). While real incomes in Africa have risen ten-fold whereas they have been stagnated in CAR. Central African Republic has also seen no improvement on almost every indicator from health, education and governance to the ease of doing business. Causes of Poverty in CAR There are many factors that ignite the fire of poverty in CAR in which poor agriculture conditions and lack of adequate medical facilities are the main causes. Agriculture is the backbone of its unstable economy. Also the hospitals and medical institutes of CAR are unable in providing the good and necessary medical facilities. The other main problems with development are the poor transportation infrastructure, and the weak internal and international marketing system (US department, 1989). Lack of education and awareness is also one factor that hinders the development of the country by coming in the path of an individuals social life. Female are still kept apart with the bookish knowledge. Only 32% of the total female population was able to make it up to secondary school where as the adult literacy rate for men is 54% (UN, 2009). Economy Central African Republic has one of the least developed economies in the world. The GDP per capita here in 2008 is only $700 (CIA, 2008 est.). A major factor behind its bad economy is its landlocked position and misdirected macroeconomic policies of government. Income distribution is unequal throughout the nation. Although it receives grants from France and other international communities but its only enough to meet the humanitarian needs (Barro, Robert J., 1995).   Ã‚  Ã‚  Ã‚  Ã‚  GDP growth slowed to an estimated 2.2% in 2008 from 3.7% in 2007. The economy suffered a number of shocks that depressed activity and led to the disappointing result electricity outages, a plunge in timber and diamond exports, higher international food and fuel prices, and continuing social tensionsInvalid source specified.. Agricultural Conditions The Central African Republics economy is dominated by the cultivation and sale of food crops such as yams, cassava, peanuts, maize, sorghum, millet, sesame, and plantains. The most important export of the CAR is diamond that accounts 40-55% of export revenues, but an estimated 30-50% of the diamonds produced each year leave the country clandestinely. The shares of the three agricultural value-added sectors in Sub-Saharan African countries consist of 28% for export crops, 45% for cereals, and 27% for other agriculture (Janvry and Sadoulet, 2001, pp. 1). Poor economic development hinders the export trade, and the location of this country far from the coast. Most of the rural and urban women are indulge in the work of transformation of some food crops into alcoholic drinks like sorghum beer or hard liquor and derive considerable income from the sale of these drinks. In CAR, subsistence farming prevails. Only 4% of the arable land is cultivated each year and more than one in three childr en under the age of five are chronically malnourished.   Ã‚  Ã‚  Ã‚  Ã‚  The importance of food crops over exported cash crops is illustrated by the fact that the total production of cassava, the staple food of most Central Africans, ranges between 200,000 and 300,000 tons a year, while the production of cotton, the principal exported cash crop, ranges from c. 25,000 to 45,000 tons a year. Food crops does not constitute in the principal cash crops of the country because Central Africans derive far more income from the periodic sale of surplus food crops than from exported cash crops such as cotton or coffee. The Sub-Saharan African economies are remarkable for the large share of agriculture in GDP (47%), and hence the large potential aggregate growth effects derived from technological change in agriculture. For the rural poor, income derived from agriculture is 67% of total income, with the remaining 32% derived from wage earnings. For them, an agricultural commodity makes a large share (72%) of total consumption. Children and Women Sector The conditions of children and women are very pathetic in this country. They are often deprived of their rights. Children are unable to get proper and required education. They find it very hard to get even primary education. Women also work very hard in their homes to add some income for their family. Also one of the biggest evils that are in common practice here is children and women trafficking. The CAR is an origin for trafficking in children and women. Children are trafficked to Cameroon. Children from Chad, Nigeria, and Sudan are reportedly trafficked to the Central African Republic. There is also internal trafficking in the Central African Republic. According to the recent study of the United Nations Childrens Fund trafficking in African women and children that are done for forced prostitution or labor is caused due to war, poverty, and flawed or nonexistent birth registration systems. â€Å"Poverty aggravates already desperate conditions caused by conflict, discrimination, and repression, and unregistered children are easy to move between countries because they never formally acquire a nationality.†(Fowler, 2004) The study also found that the population which is most vulnerable to trafficking in Africa consist of 3.3 million refugees and 12.7 million internally displaced persons (UNICEF)(Fowler, 2004). Education system in Central African Republic is also a major factor that encourages poverty. Also only 50% of the children in CAR are enrolled in primary school. The other 50% lacks in opportunity to receive even the most basic education because of violence, poverty, or also because there are no teachers, facilities or materials with which to operate a school. With its Poverty Reduction Strategy, the Central African government has announced several goals that are intended to focus efforts in the education sector that includes achieving universal primary education, improving the quality of education in general, developing literacy programs, developing short professional training courses and professionalizing higher education. Also this sector is so important that it is also considered as one of the eight goals in Millennium Development Goals. The obstacles are many, but the past has shown that humanitarian organizations working in tandem with the government can achieve positive results (United Nations, 2008). Health Sector Life expectancy of its meager population 4.3 million ranges from 43.46 to 43.62 years. The major threat to the people there is HIV. People are at very high degree of risk to many other fatal diseases like malaria, hepatitis-A, malaria and rabies. As estimated in 2004 there were fewer than 3 physicians and 9 nurses per 100,000 people (Whiteside, A., 2002)   Ã‚  Ã‚  Ã‚  Ã‚  According to United Nations approximately 11% of the population aged between 15 and 49 is HIV positive. About 13.5% of its population is at risk of AIDS. During 2003 approximately 23000 adults and children died of HIV/AIDS epidemic. Also by the end of 2003 about 110,000 children lost one or both of their parents to AIDS. The main reason that led to the deterioration of basic health services in the country is political instability and civil conflicts, thus weakening the national response to this epidemic (WHO, 2005).   Ã‚  Ã‚  Ã‚  Ã‚  As the people Central African Republic earns a very low-income it is very hard for them to meet the very high cost of drugs that is needed for their treatment. There are concerns about the adequate availability of antiretroviral drugs and financial capacity to sustain the cost of providing antiretroviral therapy free of user charges in the public sector. Additional support is required for decentralization process to ensure that services are accessible at the district level.   Ã‚  Ã‚  Ã‚  Ã‚  During 2004-2005 WHO estimated that CAR requires between US$ 31.1 million and US$ 32.3 million to scale up antiretroviral therapy to reach the WHO â€Å"3 by 5† treatment target of 19 500 people (WHO, 2005).   Ã‚  Ã‚  Ã‚  Ã‚  A successful proposal which focuses on scaling up voluntary counseling and testing services, preventing mother-to-child transmission and improving access to antiretroviral therapy was submitted to the Global Fund by the Central African Republic to fight AIDS, Tuberculosis and Malaria with a total funding request of US$ 25 million. Conclusion   Ã‚  Ã‚  Ã‚  Ã‚  It can be seen that Central African Republic is facing many problems these days related from agriculture to health care system. As we can see that the major problem in front of the countrys growth is the HIV AIDS. Also government needs to come up with more effective development programs. Government and the localities should think to improve the backbone of its economy. Despite having some of the most fertile land in Africa, the agricultural sector in the Central African Republic (CAR) has languished severely. A combination of insecurity, a lack in security, trade routes, of infrastructure, tools and skills has conspired against a country otherwise blessed with natural resources. Bibliography V. T. LeVine, Political Leadership in Africa (1967). P. Kalck, Central African Republic: Economy (tr. 1971). Barro, Robert J., Inflation and Economic Growth (October 1995). NBER Working Paper No. W5326. Available at SSRN: http://ssrn.com/abstract=225390 Jonathan Fowler, â€Å"UNICEF: Human Trafficking in Africa Fueled by War, Economic Hardship, and Lack of Birth Registration,† Associated Press, 23 April 2004. UN Statement, June 1998 -signed by the heads of all UN agencies.

Wednesday, November 13, 2019

Buddhism :: essays research papers

Buddhism: Things I Find Interesting As I was reading the selected portions of the book for this chapter, I came across a few things that I found interesting. At first I did not catch them, but after I went back and reread the selections, I found these things, that I thought were intriguing. Buddhism is supposedly a non-theistic religion. However, in the reading titled "The Majjhim-Nikaya: Questions Which Lend Not to Edification" (5.1) and in "Realizing the Four Noble Truths" (5.3, the Buddha is continually referred to as "The Blessed One". If he is only a man, why is he referred to in such a way? Other Buddhists have the potential to become Buddhas, so would they also be referred to as "Blessed One"? If this Buddha was not the first, and not the last Buddha, why are other Buddhas also not referred to as "Blessed ones"? In my opinion, if this Buddha is not considered to be a god, then he should at least be referred to as "One of the Blessed Ones" instead of "The Blessed One". Another concept that I found interesting was the idea of duality that was discussed in "The Majjhima-Nikaya: Questions Which Lend Not to Edification" (5.1). I do not quite understand it, so I was intrigued it. The selection says that the soul and body are identical, and then that the soul and body are separate. It says that the world is finite and that the world is infinite. It also says that the world is eternal and then that it is not. How can these things be? I do not understand how the saint can both exist and not exist after death. I think that in my worldview, things such as these concepts are not present. I see ideas such as the world, and the body and soul as one way or the other.

Monday, November 11, 2019

Dick Spencer Case Analysis Essay

Dick Spencer In this case Dick Spencer, a well-known employee of an aluminum producing company worked his way up the ranks from being a successful salesman to a plant manager. However through his transition of roles Dick experienced some pitfalls. In the following paragraphs I will discuss the main contributing factors to Dick’s success as a salesman and the issues he faced as a plant manager and provide informed alternative solutions. Dick knew the business well which played a vital role in his success as a salesman. Fresh out of college and with a Master’s degree in Business Administration attributed to his knowledge of the business world. According to Foster-Pedley (2013), individuals with a MBA apply their new expertise and fresh talents in their jobs and towards the advancement of the organization. â€Å"They use resources better, applying their imagination and creativity more effectively, and dare to transform and make progress† (Foster-Pedley, 2013, p. 13). Individuals who attained a MBA credit their promotion, field change or pay increase to the degree (Ainsworth, 1995). We’ve all heard the adage,† knowledge is power† and Dick certainly had the potential and prevailed as a salesman. Dick had the ability to sale and according to his coworkers he was personable which amplified his sales ability. Effectively communicating and connecting with customers is an important aspect of the buyer-seller relationship (Hung, Lin, 2013). Whether it’s on a personal level or professional level making that relation is the key. Through his easy to talk to personality, Dick built relationships and gained trust. Research indicates â€Å"higher levels of trust are expected to result in more positive attitudes, higher levels of cooperation and other forms of workplace behavior, and superior levels of performance† (Dirks, Ferrin, 2001). Additionally in order to sale a product you have to know your product. Product knowledge directly correlates with how customers will respond to your product (Sangtani, Murshed, 2013). Once you’ve established a connection with the customer you want to listen and understand your customer’s needs. Having product knowledge is important so you can identify what your product(s) can do to serve your customer’s needs. According to Feiertag (2006), â€Å"It is just not enough to simply mention the feature; it needs to be presented as a value to the prospective buyer.† Successful individuals working in sales are enthusiastic and highly motived about  se lling their product (Stevenson, 2004). Dick was able to signed several large contracts by applying these fundamental sales skills. Dick was confident in his abilities to get the job done. Shortly after being employed Dick secured a single, large contract deal, placing him high on the sales leader board. Dick was confident in his abilities and took pride in getting the job done. It’s important to have confidence at work to professionally grow and advance your career. Dick’s confidence as a salesman later helped him get a management position. Dick enjoyed being a salesman and was pretty good at it. The study performed by Gyllensten and Palmer (2014), found participates were happier on the job and outside of work when their confidence levels increased. They also reported improvements in employees completing their work duties and a willingness to make decisions (Gyllensten and Palmer, 2014). Again we can also give credit to Dick’s MBA degree as providing self-assurance in his job performance abilities (Simpson, 2005). Confidence gives you the courage to do things you normally wouldn’t feel comfortable doing. In sales there are plenty of circumstances where the ordinary sales pitch just won’t seal the deal. Dick was also very committed to the job which reflected in his sales volume. In a review of the research on organization commitment and job performance, researchers identified there is a direct correlation in commitment to the job and achieving favorable results (Myer, Paunonen, Gellatly, Goffin, Jackson, 1989). Consistent with this prospective, Shore and M artin (1989) found that â€Å"better performance was shown by employees with higher commitments.† Dick was so committed to the job that it cost him his first marriage. He was serious about reaching his sales goals and each of these factors previously described played a part in his success as a salesman. However after a few years as a salesman, Dick wasn’t getting the satisfaction from the job as he had in the past. Therefore he requested a transfer out of the sales department and into a management role. Dick was named plant manager at Modrow. He came in at a hectic time when a lot of changes were being implemented. In his efforts to familiarize himself with the daily operations, Dick first struggle as a manager was micro-managing. He had his hand in everything possible which made his employees feel very un-easy. According to Dr. White, Dick possessed many symptoms of a Micromanager. For example, Dick interest in cutting costs required the expert knowledge of the accounting group. Knowing that  accounting wasn’t his strongest subject he still spent numerous hours burying himself in details that he just could not understand. Micromanagers don’t allow any decisions be made with their approval (White, 2010). Dick had also taken on too many tasks at once making it almost impossible for any of them to be implemented successfully. Being the micromanager that he was, Dick didn’t delegate tasks very well, another symptom of a micromanager (White, 2010). Another example of Dick’s micromanaging is when he insisted on changing the disposal process of scraps. When discussing this change with his foremen, Dick didn’t listen to the foreman’s thoughts as to why the change wouldn’t work. Micromanagers tend to have the mentality; it’s my way or the highway. Traditional micromanagers will completely disregard new concepts offered by their subordinates (Weyand, 1996). Work-related stress affected Dick’s job performance. It also was creating issues with his family as they felt abandoned while he spent countless hours at the office. Through trying to prove himself to leadership and to his new employees, Dick lost focus on other important matters. His management position required him to take on more responsibilities which demanded more of his time. â€Å"In order for supervisors and managers to excel and be a dynamic force in organizational life, job stress must be harnessed† (Stanley, 2014). As Dick noticed processes that needed to be changed he was unsuccessful in communicating those changes. As a manager being able to successfully communication is essential. Communication failure could potentially become a serious problem in an organization. Information is lost causing confusion reducing productivity and increases costs. In an article published by Jost (2006), he discusses several situations where failure to communicate resulted in some hefty financial costs to many companies. Dick was so focused on cutting costs that he forgot to look at the big picture. If he had effectively communicated his interest to cut costs with his supervisors and foreman, he could have potentially found a solution. Additionally, his relationships with his employees weren’t the best, which is another consequence of ineffective communication (Tunk, 2014). Now I’d like to focus on the following recommendations that could resolve Dick’s  micro-managing style, work-related s tress, and ineffective communication. To address Dick’s micro-managing style, he needs to be flexible in how the job gets done. Dick can delegate tasks that can be handled without his supervision and give them ample time to complete each task. He can do this by building trust, empowering his employees to be a part of the decision making process (White, 2010). Making rounds within the plant had a negative impact on employees. They were worried about what he was up to and why he kept coming around. There was a lack of trust. His presence caused everyone to be less productive in their jobs. The second recommendation is time management to address Dick’s work-related stress. It’s important for Dick to find a balance between work and family for the sake of his marriage and kids but also for his well-being. If Dick continues operating this way it could potentially lead to some serious health problems (Heikkia et al., 2013). To help him find this balance he needs to reevaluate what’s most important to him. Review his job duties that need to be completed and prioritize them. Identify the tasks that can be done by someone else and delegate those tasks (McDonald & Hucheson, 1998). Dick should also try to engage in outside activity that requires his complete attention. Research supports â€Å"that a psychological detachment from work effectively mitigates some of the negative effects of work-family conflict† (Moreno et al., 2009). My last recommendation is improving his communication skills. Dick was not successful in trying to implement a change in the process to discard scrap siding. The keys to effective communication is listening and learning. Effective communication improves work performance and productivity within the business. It also builds trust and creates transparency which is something Dick is currently lacking within the Modrow plant. Communication promotes collaborative work where everyone has an opportunity to share information (Tunk, 2014). If Dick were to implement these few changes I believe he would start reaping the benefits of his management role and overall increase morale at the plant. References Ainsworth, M., & Morley, C. (1995). The Value of Management Education: Views of Graduates on the Benefits of Doing a MBA. Higher Education, 30(2), 175-87. Dirks, K. T., & Ferrin, D. L. (2001). The Role of Trust in Organizational Settings. Organization Science, (4), 450. doi:10.2307/3085982. Feiertag, H. (2006). Product knowledge is key to improving group sales. Hotel & Motel Management, 221(8), 10. Foster-Pedley, J. (2013). Older and maybe just a little bit wiser.. Finweek, 12-13. Gyllensten, K., & Palmer, S. (2014). Increased employee confidence: A benefit of coaching. Coaching Psychologist, 10(1), 36-39. Heikkilà ¤, K., Fransson, E. I., Nyberg, S. T., Zins, M., Westerlund, H., Westerholm, P., & †¦ Kivimà ¤ki, M. (2013). Job strain and health-related lifestyle: Findings from an individual-participant meta-analysis of 118,000 working adults. American Journal Of Public Health, 103(11), 2090-2097. Hung, K., & Lin, C. (2013). More communication is not always better? The interplay between effective communication and interpersonal conflict in influencing satisfaction. Industrial Marketing Management, 42(8), 1223-1232. doi:10.1016/j.indmarman.2013.05.002. Jost, A. C. (2006). What We’ve Got Here Is†¦ FAILURE to Communicate. (cover story). Journal Of The Quality Assurance Institute, 20(4), 2-5. McDonald, B. D., & Hutcheson, D. (1998). Balancing work, family, work, self and work hurts employees. Business Press, 10(48), 29. Meyer, J. P., Paunonen, S. V., Gellatly, I. R., Goffin, R. D., & Jackson, D. N. (1989). Organizational Commitment and Job Performance: It’s the Nature of the Commitment That Counts. Journal Of Applied Psychology, 74(1), 152. Moreno-Jimà ©nez, B., Mayo, M., Sanz-Vergel, A., Geurts, S., Rodrà ­guez-Muà ±oz, A., & Garrosa, E. (2009). Effects of work-family conflict on employees’ well-being: The moderating role of recovery strategies. Journal Of Occupational Health Psychology, 14(4), 427-440. doi:10.1037/a0016739. Shore, L. M., & Martin, H. J. (1989). Job satisfaction and organizational commitment in relation to work performance and turnover intentions. Human Relations, 42(7), 625. Simpson, R., Sturges, J., Woods, A., & Altman, Y. (2005). Gender, Age, and the MBA: An Analysis of Extrinsic and Intrinsic Career Benefits. Journal of Management Education, 29(2), 218-247. Stanley, T. L. (2014). stress management for high achievers. Supervision, 75(5), 17. Stevenson, B. (2004). 4 Reasons Why Sales Fail. NZ Marketing Magazine, 23(1), 32-33. Tuck, P. (2014). A failure to communicate. Training Journal, 43-47. Weyand, J. (1996). Micromanagement: Outmoded or alive and well?. Management Review, 85(11), 62. White Jr., R. D. (2010). The Micromanagement Disease: Symptoms, Diagnosis, and Cure. Public Personnel Management, 39(1), 71-76.

Saturday, November 9, 2019

Chinese Dress Essay

Chinese clothing is an important part of their culture. Although China no longer dresses in their older more traditional styles, the traditional garments are still worn for holidays and ceremonies. There have been many historical changes in Chinese clothing, and the Chinese style choices vary depending on what region is being scrutinized. Hanfu is a term that includes all traditional Chinese dress worn prior to Manchurian and western influences. All Chinese citizens living under the Ming dynasty, and many dynasties prior to it adopted this style of dress. It was widely worn by all until the beginning of the seventeenth century and the start of the Quing dynasty. Hanfu, which was the widely accepted style in China for over three thousand years, consists of the Shenyi. A one-piece robe, which wraps around the body, the Shenyi is made up of different parts. The Jin, or the upper front of the body, the Jiaoling Youren, which means â€Å"crossed collars to the right† in Chinese and is the collar usually seen on the Hanfu of both men and women. The collar is only tied to the left as burial dress. The Qu and Mei are the cuffs and sleeves, and the Chang is the name of the skirt. The first recorded dynasty in China was the Shang dynasty. Although they did wear Hanfu clothing, most specifically the Shenyi, the garment worn by them was actually made of two separate pieces. The jacket, referred to as the Yi, and the skirt, or the Shang. It did not become a one-piece garment for many years. During the Shang dynasty the colors most often used for the garments were warm colors such as red and yellow. As time moved forward the Chinese began to decorate the fabrics with red and yellow designs. Different regions of the country had different norms as far as Hanfu dress. During the Zhou dynasty the western people used different styles of the Hanfu Shangyi to differentiate between classes. The differences in classes are shown through the two styles of sleeves, the broad and the narrow, and the decorations that the wearer hung from his or her belt such as pieces of jade. When the Quing dynasty took over, so did a new style of clothing. The Qipao was the traditional style for women and the Cheongsam was the style commonly worn by men. All Chinese were required to switch from the old Hanfu style of dressing or they would be killed due to the new rulers in place. The Qipao is a one-piece dress that traditionally fits loosely on the body and was designed to hide the woman’s figure. It is also sometimes referred to as the mandarin gown. In the early nineteen hundred the Qipao was updated into the dress we recognize as traditional Chinese dress today. The close fitting dress with a high neckline is now often worn as a party dress and has been copied and worn by American women as well. The Cheongsam is the men’s version of the Qipao. It is a two-piece outfit made up of a pair of simple pants and a top with a high neckline extremely similar to that of the Qipao. The Cheongsam is traditionally made in the color black, and was the formal dress worn by Chinese men up until the introduction of the western-style suit.

Wednesday, November 6, 2019

Free Essays on Battle of Chancellorsville

The Battle of Chancellorsville took place from May 1, 1863 to May 4, 1863 after the Battle of Fredericksburg and is said to be one of General Robert E. Lee’s greatest accomplishments during the Civil War. General Lee wanted to take the to fight to the enemy’s territory in Pennsylvania, to break up and capture the coalfields, and to cut off the fuel supplies for the manufacturing establishments, railways, and steamships. At the beginning of 1863 he directed the topographical engineers to prepare a detailed map from the Potomac River to the Susquehanna River. This map ended up being used at the Battle of Gettysburg. After the battle of Fredericksburg and the failure of the Mud Campaign the Union army was demoralized. Desertions were frequent and insubordination was common practice throughout the ranks. To rectify this problem President Lincoln appointed Major General J. Hooker to replace Major General Burnside as the Commander of Union Army in the Potomac. He restored forces to about 134,000 soldiers. General Hooker wanted to start a campaign to take the fight to General Lee and start the campaign towards the end of April. General Hooker sent Major General Sedgwick across the Rappahannock River with three battalions, just below Fredericksburg. General Hooker with the Fourth Corps would march up north of the Rappahannock River concealed by the forest, and cross the river Germanna and Ely Fords near Chancellorsville. Then they marched on the road leading to Fredericksburg. General Hooker was hoping to come across General Lee’s rear flank and force him to retreat towards Richmond. Hooker was then planning on giving chase to the Confederate Army. On April 13, 1863, General Hooker started executing his plan by sending an advance cavalry lead by General Stoneman with 10,000 troops to cross the Rappahannock River at Kelly’s Ford. To attack his supply base and railroad deep behind Confederate Army lines at Gordonsvill... Free Essays on Battle of Chancellorsville Free Essays on Battle of Chancellorsville The Battle of Chancellorsville took place from May 1, 1863 to May 4, 1863 after the Battle of Fredericksburg and is said to be one of General Robert E. Lee’s greatest accomplishments during the Civil War. General Lee wanted to take the to fight to the enemy’s territory in Pennsylvania, to break up and capture the coalfields, and to cut off the fuel supplies for the manufacturing establishments, railways, and steamships. At the beginning of 1863 he directed the topographical engineers to prepare a detailed map from the Potomac River to the Susquehanna River. This map ended up being used at the Battle of Gettysburg. After the battle of Fredericksburg and the failure of the Mud Campaign the Union army was demoralized. Desertions were frequent and insubordination was common practice throughout the ranks. To rectify this problem President Lincoln appointed Major General J. Hooker to replace Major General Burnside as the Commander of Union Army in the Potomac. He restored forces to about 134,000 soldiers. General Hooker wanted to start a campaign to take the fight to General Lee and start the campaign towards the end of April. General Hooker sent Major General Sedgwick across the Rappahannock River with three battalions, just below Fredericksburg. General Hooker with the Fourth Corps would march up north of the Rappahannock River concealed by the forest, and cross the river Germanna and Ely Fords near Chancellorsville. Then they marched on the road leading to Fredericksburg. General Hooker was hoping to come across General Lee’s rear flank and force him to retreat towards Richmond. Hooker was then planning on giving chase to the Confederate Army. On April 13, 1863, General Hooker started executing his plan by sending an advance cavalry lead by General Stoneman with 10,000 troops to cross the Rappahannock River at Kelly’s Ford. To attack his supply base and railroad deep behind Confederate Army lines at Gordonsvill...

Monday, November 4, 2019

Sources of Clean Energy Essay Example | Topics and Well Written Essays - 750 words

Sources of Clean Energy - Essay Example So the point is that the only way to avoid the global power crisis in future is to follow the policy of natural recourses saving and to use renewable sources of energy, such as solar energy, geothermal energy, wind power, water power, and biofuel. So renewable energy is 'energy derived from resources that are regenerative or for all practical purposes can not be depleted2'. Wind - is one of the renewable energy sources. The wind is considered by experts as one of the most perspective energy sources, capable to replace not only traditional sources, but also nuclear power. Production of the electric power by means of wind has a number of advantages: But as well as in other sources of energy usage, we can find some negative aspects in usage of wind power. The essential defect of a wind power is its intermittency. Moreover the usage of wind power may lead to a kind of environmental pollution, which existence and importance are often undervalued. It is a noise pollution. In Ohio one of the leading American companies has constructed the world's largest wind power plant with rating power 10 MW. This massive construction with the general height over hundred meters has worked just for some days and after has been sold for demolition at the price of 10 dollars per ton. The thing is that the life in radius of several kilometers has become just impossible. And question is not only in audible noise and vibrations. There is a very strong infrasonic, low frequency component in this noise. The point is that an infrasound on the frequency of 7 Hz conterminous with an alpha rhythm of a human's brain at sufficient intensity is very harmful. It may cause the strongest health disorders. Moreover the usage of wind turbines may result desertification of the grounds and in this case animals would have to leave their traditional areas of living. So the biodiversity may be decreased. Solar Power The leading clean energy source is the solar power. The energy of a solar radiation coming on the surface of our planet, almost in 40 times exceeds all energy consumed by the mankind. Every second the Sun gives the Earth 80 thousand billion kilowatt of energy, that in some thousand times more, than all the power stations of the world produce all together.Now mankind use only insignificant part of a solar energy, as existing solar batteries have rather low efficiency and are very expensive to produce. However we should not refuse from this practically inexhaustible source of clean energy. According to the statements of experts, this source could cover all needs of mankind for energy thousand years forward. The solar power is the most powerful and accessible of all kinds of non-traditional, renewable energy sources. Sunlight is not only inexhaustible, but also absolutely clean energy source having enormous energy potential. Nevertheless the usage of solar power also has own disadvanta ges.Solar energy is distributed on the big area, or in other words, has low density. That is why any plant for direct use of solar

Saturday, November 2, 2019

The Immigration Experience of Vietnamese Americans Essay

The Immigration Experience of Vietnamese Americans - Essay Example However these people have strongly come back and made their place in the United States of America. This article would further discuss the Vietnamese Americans in detail. In 1975 Vietnam was undergoing a war which came to an end. This war even after ending left its effect on the Vietnamese people. The government which was supporting the people of Vietnam was now about to change and this created an atmosphere of insecurity for the Vietnamese people. In 1975 the first group of immigrants of Vietnamese people left their country. These people at first migrated to the refugee centers developed by the government of United States. However after some legislations were introduced these refugees were allowed to properly live in America. The era of immigrants did not end here as the new government started taking strict actions against the people of Vietnam. This further encouraged the Vietnamese people to leave their own land and migrate to America. As more and more immigrants were fleeing to United States the state restricted the entry of these people after a certain limit. However this step taken by the government of America harmed the people of Vietnam and f urther helped the new government in Vietnam to take proper actions against them. The journey of immigration for the Vietnamese people did not end here as living on the soil of America was more difficult then expected by them. The people belonging to Vietnam were equipped with a strong culture which was to be followed. This culture had rules which were not seen by the Americans previously. And this culture only made their immigration and the settling journey harder. The culture of Vietnamese people did not allow their women to work but after coming to America the men had to let go off their culture and let their women work to earn a living. This was another blow to the Vietnamese people for settling. However the people of Vietnam did not lose hope and kept on with their struggle. These people not only faced hardships economically but also socially. The children belonging to Vietnam were not able to properly talk in English and found it difficult when conversing with a native. On the other hand the education given in America was through the medium of English and this factor made the children of Vietnam leave behind in the education sector also. The people of Vietnam had strong differences in culture to that of the natives and this posed another difficulty for these Vietnams to settle in America. The people of Vietnam were not only faced by the problem of differing cultures but also with the problem of discrimination. The natives did not like the arrival of these immigrants from Vietnam and this made it even more difficult for the Vietnamese people to settle in America. The natives did not like the Vietnamese people due to the fact that they were taking over the economics and the industrial unit of America. Thus this posed another problem to the Vietnamese people. However after a series of hardships the Vietnamese people grew in numbers and took over America. These people after a long time started to settle properly on the soil of America. The children of Vietnamese people were given special instructions by their parents to learn English. They have even arranged special classes of English for their children. This has helped the children of Vietnam to increase their knowledge and enhance their educational level.

Thursday, October 31, 2019

Programming III ( Advanced ) Assignment Example | Topics and Well Written Essays - 750 words

Programming III ( Advanced ) - Assignment Example ation and deserialization is independent of the platform used, that is, data serialized on one machine is able to be deserialized on a completely different machine with a different platform (DAnjou, 2005). Serialization in java has been used mainly to facilitate communication between different machines, for persistence, deep copying, caching and cross java virtual machine synchronization. The use of serialization is advantageous since it allows for refactoring , it is customizable and easy to use, allows encryption, compression and authentication thus facilitating secure computing and lastly, the mechanism allows interchange of libraries between java and other programing languages such as C++ among other advantages. Despite the above stated functions and advantages of serialization in java, there are too many disadvantages associated with serialization. Serialization offers significant overhead thus it cannot be used with large objects. Also, the serialization facilitates increased memory requirements for a system. The use of data serialization also leads to breakage of identity of an objects, and invasion of the programming model. To address this disadvantages and limitations of serialization, an input/output process mechanism has been introduce. This mechanism is referred to as Externalization. Not only is Externalization used to eliminate the limitations of serializations, but also in some cases, it is exclusively required. Externalization refers to the process in which externalizable interface is implemented to facilitates persistence and restoring of the object. Basically, the externizable interface works by extending the serializable interface. Moreover, the externizable interface facilitates definition of custom rules and also, definition of customizable serialization mechanism. Thus, it can be easily said that externalization is an extension of serialization. The process of using Externalization is similar to the implementation process of

Tuesday, October 29, 2019

To Kill a Mockingbird Book Response Essay Example for Free

To Kill a Mockingbird Book Response Essay To Kill a Mockingbird is one the most enduring stories to be told. One of the reasons for its appeal is that it brings to light social issues that remain as relevant to day as they have been when Harper Lee (1988) wrote it. One of the most interesting facets of the story is that it is told from the perspective of a child that challenges adult readers claim to maturity and wisdom. The summation of Tom’s case is expresses the tragedy of the story: â€Å"Atticus had used every tool available to free men to save Tom Robinson, but in the secret courts of mens hearts Atticus had no case. Tom was a dead man the minute Mayella Ewell opened her mouth and screamed. (pp. 244-245). This realization is the realization that the trial revealed that despite the illustration of Maycomb, Alabama as a sleepy, tired town, there were significant social conflicts just beneath its social faà §ade. As a classroom material, the book’s coming of age style allows younger readers to relate effectively with the story. Since the setting maybe alien to students, there should effort to explain the social relevance of the issues and its possible application today. There should be emphasis that Lee goes beyond race as a tool of discrimination and seeks to address censure for differences with mainstream society. Lee’s work is an inspiration to other writers in engaging their audience. The presentation of various perspectives on the issue without changing the voice of the story also allows readers to easily place themselves in not just the narrator’s shoes but keep touch with their own. After reading the book, one has the realization of the power of discrimination and social exclusion. Moreover, that regardless of how enlightened or placid people are, these issues always cause significant rift and conflict in the community. Reference Lee, Harper (1988). To Kill a Mockingbird. New York: Grand Central Publishing Lee, Harper (2008). To Kill a Mockingbird. Book Rags. Retrieved on February 20, 2008, from http://www.bookrags.com/To_Kill_a_Mockingbird

Sunday, October 27, 2019

The Polymerase Chain Reaction Pcr

The Polymerase Chain Reaction Pcr The polymerase chain reaction was first developed in 1983 by Kary Mullis. This reaction is commonly used in molecular biology to amplify and generate thousands to millions of copies of specific DNA sequences across several orders of magnitude (4-1). It relies on thermal cycling, consisting of cycles of denaturation, primer (short DNA fragment) annealing and primer extension (4-7). PCR can also be used for the analysis of RNA sequences and to qualitatively detect RNA expression levels through creation of complementary DNA (cDNA) transcripts from RNA by use of reverse transcriptase. This technique is called reverse transcription-PCR (RT-PCR) (5-2). Although PCR and RT-PCR have revolutionized many areas of biomedical science, they are not suitable for the quantitative analysis of analysis of samples. Hence, real-time or quantitative PCR (qPCR) techniques need to be employed (5-8, 5-9). RT- qPCR distinguishes itself from other methods available for gene expression, such as northern-blot analysis, ribonuclease (RNase) protection assay and competitive RT-PCR, in term of accuracy, sensitivity and fast results (2,6). RT-qPCR does not required post-amplification manipulation and it can produce quantitative data with wide dynamic range of detection (7 to 8 logs). In addition, RT-qPCR assay is 10,000 to 100,000-fold more sensitive than RNase protection assays and 1000-fold more sensitive than dot blot hybridization (3). RT- qPCR also can even detect a single copy of a specific transcript and can reliably detect gene expression differences as small as 23% between samples (3-6, 3-7). Furthermore, it has lower coefficients variation (cv; TaqMan at 24%; SYBR Green at 14.2%) than end point assays such as probe hybridization and band densitometry (45.1%; 44.9% respectively) (3-8). RT- qPCR can differentiate between messenger RNAs (mRNAs) with almost identical sequences and requires much less RNA template than other methods of gene expression analysis. Because of this, RT- qPCR has established itself as the gold standard for the detection and quantification of RNA targets (1-2,2). Furthermore, it is firmly established as a mainstream research technology (1-3). However, the major disadvantage of RT-qPCR is that required expensive equipment and reagents (3). The principle of RT-qPCR is straight forward: following the reverse transcription of RNA in to cDNA, it needs an appropriate detection chemistry to dete ct the presence of PCR products, an instrument to monitor the amplification in real-time and compatible software for quantitative analysis. RT- qPCR is characterized by the point in time during cycling when a PCR product amplification is first detected (Figure 1, 1). A direct relationship between the starting copy number of the nucleic acid target and the time required to observe fluorosence increasing. Nowadays, there are four fluorescent DNA probes available for RT-qPCR detection of PCR products: TaqMan, SYBR Green, Molecular Beacons, and Scorpions. All of them generate a florescent signal to allow the detection of PCR products. While the TaqMan probes, SYBR Green, Molecular Beacons, and Scorpions generation of fluorescence depend on Forster Resonance Energy Transfer (FRET) coupling of the dye molecule and a quencher moiety to the oligonucleotide substrates, the SYBR Green dye simply emits its fluorescent signal by binding to the double-strand DNA in solution (5-34). As RT-qPCR has extremely high sensitivity and reproducibility, in depth understanding of normalization techniques is imperative for accurate conclusions (6). Normalization of gene expression data is an essential component of a reliable RT-qPCR assay and it is used to control for error between samples (7,3). This error could be introduced at one or more stages throughout the experimental protocol; (input sample, RNA extraction, etc.) however, there are many strategies to control this error ( please, read the discussion section strategies for more details). Currently, internal control genes, which are often referred to as housekeeping genes, are most frequently used to normalize the messenger RNA (mRNA) fraction. This housekeeping gene should remain constant in the tissues on cells under investigation, or in response to experimental treatment (8, 8-69, 8-70, 6,7). In addition, the ideal housekeeping genes should by stably expressed, and their abundances should show strong correlation w ith mRNA total amounts present in the samples (8). Consequently, normalization against a single housekeeping gene in not acceptable and can falsely bias results unless the researchers present clear evidence for the reviewers that confirms its invariant expressions conditions described (3,8-71,8-73). In this study we carried out an evaluation the gene expression of three commonly used housekeeping genes (GAPDH, ÃŽÂ ²-action, ALAS1) in three different cell lines which are derived from T-cell leukemia, B-cell lymphoma and myeloid leukemia, using RT-qPCR as an analytical tool. Our goal was to recognize a housekeeping gene with minimal variability under different experimental conditions. Materials and Methods: Samples, RNA handling and isolation: Cell line pellets (5-10X106 cells) which have been frozen in 0.5 ml TRIsure Reagent (Bioline code BIO-38033). Cell lines are: Jurkat, CEM-C7 and MOLT-4 (all T-cell leukemia-derived); SKW 6.4, BJAB and JeKo-1 (all B-cell lymphoma-derived); and HL-60, NB4 and K562 (all derived from myeloid leukemia). RNA was isolated from cell pellets using Trizol procedure. However, isolating and handling RNA ask for special precautions because naked RNA is highly susceptible to degradation by ribonucleases (RNases) (1). RNases are found everywhere and they are very stable and active enzymes that do not require metal ion co-factors to function and can maintain activity even after prolonged autoclaving or boiling. So that, all equipment and reagents should be treated to inactive RNases prior to use. Wearing gloves while handling reagents and RNA samples, changing gloves frequently, keeping tubes closed whenever possible and keeping isolated RNA on ice when aliquots are pipetted for downstream applications could prevent RNase contamination. We are used sterile, disposable plastic ware and they were RNase-free and do not require pretreatment to inactive RNAses (8-46,8-47). The quantity of the isolated RNA was determined by nanodrop spectrophotometry (absorbance at 260 nm of a 40ÂÂ µg/ml soluti on of RNA is 1.0) using nuclease-free water as a blank. A sample was reserved for quality assurance (see below) and the remainder was stored at -80 degree centigrade for one week. Gel electrophoresis (quality assurance of RNA) : The quality of the isolated RNA was verified by agarose gel electrophoresis. The gel was run at 100V for 30 minutes and photograph under UV transillumination. DNA digestion: A DNase digestion step was performed as a precaution using RQ1 RNase-free DNase kit although the TRIzol method generally results in RNA which is essentially free from genomic DNA (2). A sample was reserved for reverse transcription (see below) and the remainder was stored at -80 degree centigrade. Reverse transcription: 11 ÂÂ µL of DNase-treated sample was reverse transcripted, using Superscript II reverse transcriptase, to complementary (cDNA) by random hexamer priming.